發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-14 瀏覽量：529
摘要
? ? ? 目的：鑒定一名腹瀉患者的廣泛耐藥銅綠假單胞菌。材料與方法：采用紙片擴(kuò)散法檢測(cè)抗菌藥物敏感性。對(duì)銅綠假單胞菌基因組進(jìn)行測(cè)序，以確定毒力、抗生素耐藥性和原噬菌體編碼基因。結(jié)果：P。銅綠假單胞菌對(duì)抗生素有廣泛的耐藥性。銅綠假單胞菌的基因組分析揭示了76個(gè)與抗微生物耐藥性、外源性降解和三型分泌系統(tǒng)相關(guān)的基因。結(jié)論：這是首次報(bào)道與銅綠假單胞菌相關(guān)的腹瀉。由于沒有發(fā)現(xiàn)其他生物體，作者認(rèn)為患者因接觸抗生素而出現(xiàn)微生態(tài)失調(diào)，導(dǎo)致抗生素相關(guān)性腹瀉。病原體表達(dá)的體內(nèi)毒性可能與T3SS有關(guān)。
Abstract
Aim: To characterize extensively drug-resistant Pseudomonas aeruginosa from a patient with diarrhea. Materials & methods: Antimicrobial susceptibility was tested by the disk diffusion method. The P. aeruginosa genome was sequenced to identify virulence, antibiotic resistance and prophages encoding genes. Results:P. aeruginosa had a wide spectrum of resistance to antibiotics. Genomic analysis of P. aeruginosa revealed 76 genes associated with antimicrobial resistance, xenobiotic degradation and the type three secretion system. Conclusion: This is the first report on diarrhea associated with P. aeruginosa. Since no other organism was identified, the authors assume that the patient had dysbiosis due to antibiotic exposure, leading to antibiotic-associated diarrhea. The in vivo toxicity expressed by the pathogen may be associated with T3SS.
https://www.futuremedicine.com/doi/abs/10.2217/fmb-2022-0140