發(fā)布者：抗性基因網(wǎng) 時間：2023-06-12 瀏覽量：453
摘要
? ? ? 抗微生物耐藥性在全球范圍內(nèi)構(gòu)成嚴(yán)重的社會經(jīng)濟和生態(tài)威脅，需要創(chuàng)新的監(jiān)測方法。在分析博茨瓦納帕拉比農(nóng)村社區(qū)的土壤、動物糞便、池水、廢水和蔬菜樣本以及環(huán)境時，考慮了一個健康概念。培養(yǎng)依賴性和下一代測序方法被用于細(xì)菌和抗生素耐藥性基因的表征。觀察到活的潛在致病菌表現(xiàn)出與各種臨床相關(guān)抗生素相對應(yīng)的多種耐藥性。下一代序列分析還揭示了與臨床上重要類別的抗生素（β-內(nèi)酰胺、氨基糖苷、四環(huán)素、磷霉素、甲氧芐啶、磺酰胺、喹諾酮、大環(huán)內(nèi)酯和氯霉素）相對應(yīng)的細(xì)菌群落和獲得性抗生素耐藥性基因的發(fā)生和多樣性。在一個農(nóng)村社區(qū)的池塘水中觀察到β-內(nèi)酰胺酶編碼（bla）基因的高度多樣性。從群落和環(huán)境樣本的宏基因組學(xué)序列中發(fā)現(xiàn)，γ-變形菌門是最豐富的。從全基因組序列數(shù)據(jù)中鑒定了編碼沙雷氏菌DM6的多藥耐藥性和重金屬耐藥性。這項研究為下一代測序在中低收入國家的應(yīng)用提供了概念驗證，并支持“一個健康”對抗菌藥物耐藥性的監(jiān)測。所產(chǎn)生的數(shù)據(jù)有助于抗菌藥物管理計劃和抗菌藥物耐藥性國家行動計劃的實施。
Abstract
Antimicrobial resistance poses serious socioeconomic and ecological threat globally, warranting innovative approaches for surveillance and monitoring. One Health concept was considered in the analysis of soil, animal feces, pond water, wastewater and vegetable samples from a rural community and the environment in Palapye, Botswana. Culture-dependent and next-generation sequencing approaches were used for characterization of bacteria and antibiotic resistance genes. Viable potentially pathogenic bacteria exhibiting multi-drug resistance profiles corresponding to various clinically relevant antibiotics were observed. Next-generation sequence analysis also reveals occurrence and diversity of bacterial communities and acquired antibiotic resistance genes corresponding to clinically important classes of antibiotics (beta-lactam, aminoglycoside, tetracycline, fosfomycin, trimethoprim, sulfonamide, quinolone, macrolide, and phenicol). High diversity was observed for beta-lactamase encoding (bla) genes from pond water in a rural community. Phylum gammaproteobacteria was revealed as the most abundant from metagenomics sequences of both community and environment samples. Multi-drug and heavy metal resistance encoding Serratia mercensens DM6 was identified from whole genome sequence data. This study serves as proof-of-concept for the application of next-generation sequencing in low- and middle-income countries and supports the One Health surveillance of antimicrobial resistance. The data generated can contribute towards antimicrobial stewardship programs and implementation of National Action Plan on antimicrobial resistance.
https://www.sciencedirect.com/science/article/pii/S2772416623000037