發(fā)布者：抗性基因網(wǎng) 時間：2023-06-12 瀏覽量：435
摘要
? ? ? 抗生素耐藥菌的繁殖嚴重危害生態(tài)安全和人類健康。本研究采用黃鐵礦（FeS2，天然豐富的礦物）進行高碘酸鹽（PI）活化以消毒ARB。FeS2/PI系統(tǒng)可以在20分鐘內(nèi)對1×107CFU mL?1的卡那霉素抗性大腸桿菌進行消毒，使其低于檢測極限。在pH為3至9、離子強度為0至300mM、懸浮液中HA（0.1–10 mg L?1）以及自來水、河水和污水等真實水樣中，都能實現(xiàn)有效的ARB滅活性能。FeS2/PI系統(tǒng)也能有效地對慶大霉素耐藥大腸桿菌和革蘭氏陽性枯草芽孢桿菌進行消毒。產(chǎn)生的反應(yīng)物種包括Fe（IV）、·O2–和·OH，會攻擊細胞膜并淹沒細胞內(nèi)防御系統(tǒng)。細胞內(nèi)的卡那霉素抗性基因在FeS2/PI系統(tǒng)中被釋放并降解。PI優(yōu)選吸附在FeS2的Fe位點上（具有較低的吸附能、較多的鍵合態(tài)占有率和較強的鍵合強度）。隨后電子云從Fe位點轉(zhuǎn)移到PI將裂解Isingle-bond-O鍵以產(chǎn)生反應(yīng)物種。此外，F(xiàn)eS2/PI系統(tǒng)還可以與砂濾系統(tǒng)相結(jié)合，有效地捕獲和消毒ARB。因此，F(xiàn)eS2/PI系統(tǒng)是一種在不同情況下滅活A(yù)RB的有前途的方法。
Abstract
The propagation of antibiotic-resistant bacteria (ARB) greatly endangers the ecological safety and human health. This study employed pyrite (FeS2, naturally abundant mineral) for periodate (PI) activation to disinfect ARB. FeS2/PI system could disinfect 1 × 107 CFU mL?1 of kanamycin-resistant E.coli below the limit of detection in 20 min. Efficient ARB inactivation performance was achieved in pH from 3 to 9, ionic strength from 0 to 300 mM, with HA (0.1–10 mg L?1) in suspension, and in real water samples including tap water, river water and sewage. FeS2/PI system could also efficiently disinfect gentamycin-resistant E.coli and Gram-positive B. subtilis. The generated reactive species including Fe(IV), ·O2– and ·OH would attack cell membrane and overwhelmed intracellular defense system. The intracellular kanamycin resistance genes in cells would be released and then degraded in FeS2/PI system. PI preferred to be adsorbed on Fe site of FeS2 (with lower adsorption energy, more occupancy of bonding state and stronger bonding strength). The subsequent transfer of electron cloud from Fe site to PI would cleave Isingle bondO bond to generate reactive species. Moreover, FeS2/PI system could also combine with sand filtration system to efficiently capture and disinfect ARB. Therefore, FeS2/PI system is a promising approach to inactivate ARB in different scenarios.
https://www.sciencedirect.com/science/article/abs/pii/S0043135422014531